Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 1. Knockdown of xbp1s mitigated MCT-induced PH Forty SD male rats were randomly assigned to four groups. MCT was administered through intraperitoneal injection, and AAV was

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Knockdown, Injection

Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 2. Knockdown of xbp1s alleviated MCT-induced vascular remodeling in the PH model (A) Representative image of HE staining, which was performed to quantify the medial wall thickness of the distal pulmonary ar-

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Knockdown, Staining

Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 3. Knockdown of xbp1s restored MCT-induced proliferation and antiapoptotic effects in the PH model (A) Representative image of immunohistochemical staining of PCNA and Ki67, which both reflect the proliferation of the detected sample. (B) Representative image of TUNEL staining. TUNEL-positive staining reflects the apoptotic state of the detected sample.

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Knockdown, Immunohistochemical staining, Staining, TUNEL Assay

Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 4. Proteins that interact with xbp1s (A) Image showing the protein interaction network focused on xbp1s. Data were predicted by the STRING database. (B) qRT-PCR results showing the relative expression of proteins predicted to interact with xbp1s. Expression was detected in PASMCs cultured

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Quantitative RT-PCR, Expressing, Cell Culture

Journal: Cell

Article Title: Inflammation Improves Glucose Homeostasis Through IKKβ-XBP1s Interaction

doi: 10.1016/j.cell.2016.10.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-phospho-XBP1s Thr48 , Covance , Raised against CRAAGSEASGT(p)PQARKRQR.

Techniques: Recombinant, Plasmid Preparation, Expressing, Sequencing, Software

Journal: Frontiers in Cell and Developmental Biology

Article Title: Obesity impacts placental function through activation of p-IRE1a-XBP1s signaling

doi: 10.3389/fcell.2023.1023327

Figure Lengend Snippet: Primers used for the QPCR.

Article Snippet: Following antibodies were used in this study: rabbit anti-p-IRE1α antibody (Thermofisher Scientific, Cat #PA1-16927, 1:2,000); rabbit anti- XBP1s antibody (ProteinTech, Cat #24868-1-AP, 1:2,000); Rabbit anti-p-Perk antibody (Thermofisher Scientific, Cat #PA5-37773, 1:2,000); and rabbit anti-Bip antibody (Cell Signaling Technology, Cat #CST-31776, 1:2,000).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Obesity impacts placental function through activation of p-IRE1a-XBP1s signaling

doi: 10.3389/fcell.2023.1023327

Figure Lengend Snippet: Obesity activates XBP1 splicing in the placenta. XBP1s specific antibody was used to label activated XBP1s. Compared to the non-obese placenta (A) , n = 10), the obese placenta exhibited increased XBP1s (B) , n = 12). Elevated XBP1s was visualized by PCR with a primer pair flanking splicing regions followed by electrophoresis (C) (unspliced, 256 bp; spliced XBP1, 230 bp) . Quantified with ImageJ on the immunocytochemically labeled sections (D) or XBP1s PCR gel images (E) showed increased XBP1s in the obese placenta. Furthermore, Reverse Transcription (RT)-QPCR demonstrated increase of XBP1s in the obese placenta (F) . Non-parametric Mann-Whitney U -test was used to compare the XBP1s between the non-obese and obese placenta. Values are mean ± SEM. N1-N12: non-obese samples; O1-O12: obese samples.

Article Snippet: Following antibodies were used in this study: rabbit anti-p-IRE1α antibody (Thermofisher Scientific, Cat #PA1-16927, 1:2,000); rabbit anti- XBP1s antibody (ProteinTech, Cat #24868-1-AP, 1:2,000); Rabbit anti-p-Perk antibody (Thermofisher Scientific, Cat #PA5-37773, 1:2,000); and rabbit anti-Bip antibody (Cell Signaling Technology, Cat #CST-31776, 1:2,000).

Techniques: Electrophoresis, Labeling, Reverse Transcription, Quantitative RT-PCR, MANN-WHITNEY

Journal: Cell reports

Article Title: MEF2A suppresses stress responses that trigger DDX41-dependent IFN production

doi: 10.1016/j.celrep.2023.112805

Figure Lengend Snippet:

Article Snippet: Rabbit anti XBP-1s (D2CF1F) , Cell Signaling Technology , RRID:AB_2687943.

Techniques: Virus, Recombinant, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Negative Control, Software